Reliability of in vitro methods used to measure intrinsic clearance of hydrophobic organic chemicals by fish: results of an international ring trial_Nichols et al._Science Hub entry_02232018

This paper describes the results of an international ring trial (round robin study) of in vitro methods used to measure chemical biotransformation in fish. Researchers at six laboratories in the U.S. and Europe evaluated six test chemicals (cyclohexyl salicylate, fenthion, 4-n-nonylphenol, deltamethrin, methoxychlor, and pyrene) using two in vitro systems (liver S9 fraction and isolated hepatocytes) derived from rainbow trout livers. This information was then evaluated to quantitatively characterize assay reliability (repeatability and reproducibility) and evaluate potential user bias associated with the methods. Additional calculations were performed using established models to estimate in vivo levels of hepatic clearance and predict the effects on chemical biotransformation on chemical bioaccumulation. The Science Hub dataset includes all information presented in the paper as figures and tables including, as appropriate, individual values used to calculate reported means and standard deviations. Also included are all datasets provided as Supplementary Data. This dataset is associated with the following publication: Nichols, J., K. Fay, M.J. Bernhard, I. Bischof, J. Davis, M. Halder, J. Hu, K. Johanning, H. Laue, D. Nabb, C. Schlechtriem, H. Segner, J. Swintek, J. Weeks, and M. Embry. Reliability of In Vitro methods used to measure intrinsic clearance of hydrophobic organic chemicals by rainbow trout: Results of an international ring trial. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 164(2): 563-575, (2018).

[
  "020:00"
]

[
  "020:095"
]

[
  "https://doi.org/10.1093/toxsci/kfy113"
]