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Found 1449 dataset(s) matching "protocol".
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Experimentation on the International Space Station has reached the stage where repeated and nuanced transcriptome studies are beginning to illuminate the structural and metabolic differences...
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Methods: RNA-seq libraries were prepared using the Illumina TruSeq RNA kit and the TrueSeq method was employed for mRNA enrichment. The libraries were quantified and samples were multiplexed in...
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With extended stays aboard the International Space Station (ISS) becoming commonplace there is a need to better understand the effects of microgravity on cardiac function. We utilized human...
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Whole seedlings of wild type (4d) and atm mutants (4d) have been analyzed after a gamma ray irradiation of 0.75h 1.5h 3h & 5h (time course). Roots of wt (4d) atm (3d) and atr (4d) mutants have...
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We report the findings of an animal experiment onboard STS-135 investigating the molecular aspects of the impact of spaceflight on retinal biology by performing differential gene expression...
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Traveling to nearby extraterrestrial objects having a reduced gravity level (partial gravity) compared to Earth's gravity is becoming a realistic objective for space agencies. The use of plants as...
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Larvae-Pupae transition flies (Drosophila) were recovered and transport for 3 days at 12-14C to arrest development until the launch site then exposed to RT (18-20C) for some hours including the...
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DNA methylation is a very important kind of epigenetic modification and participates in many biological functions. Although many previous studies have addressed various plant species the study of...
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The Rodent Research-3 (RR-3) mission was sponsored by the pharmaceutical company Eli Lilly and Co. and the Center for the Advancement of Science in Space to study the effectiveness of a potential...
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To understand how humans adapt to space environments many experiments can be conducted on astronauts while they work aboard the Space Shuttle or the International Space Station (ISS). We also need...
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The Rodent Research-3 (RR-3) mission was sponsored by the pharmaceutical company Eli Lilly and Co. and the Center for the Advancement of Science in Space to study the effectiveness of a potential...
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This dataset includes marine biological occurrence data managed by the Ocean Biodiversity Information System USA (OBIS-USA). The data were observed between 1833 and 2025-01-23. These data were...
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The general objective of the study was to determine modulation of gene expression by environmental factors specifically simulation of microgravity with a special emphasis on bone formation. For...
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The present study concerns the effects of 21 days of sustained recumbence (bedrest) on skeletal muscle gene expression. 14 male subjects participated in normoxic bedrest (NBR) with muscle...
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When germinated and grown on-board the ISS (International Space Station) plant do not exhibit abnormal structures but they do have altered growth habits and this project aims to investigate the...
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Dataset containing areas of analysis (AOA) for all NPS units used by the NPS Inventory and Monitoring Division (IMD) and the IRMA Unit database. The file geodatabase includes polygon feature...
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The wild type WS plants and the Sku5 mutant plants in the WS background were germinated on ISS or on the ground and the gene expression profiles in roots at 4 days or 8 days were established. The...
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DLD-1 and MOLT-4 cell lines were cultured in a rotating cell culture system to simulate microgravity and mRNA expression profile in comparison to Static controls. Cells were grown in 10mL rotating...
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The JAXA MHU-2 mission had two objectives: 1) To increase understanding of effects of spaceflight on the gut environment (microbiota and metabolites) and immune system using multi-omics based...
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Microarray Analysis of Space-flown Murine Thymus Tissue Reveals Changes in Gene Expression Regulating Stress and Glucocorticoid Receptors. We used microarrays to detail the gene expression of...