mRNA expression profile in DLD-1 and MOLT-4 cancer cell lines cultured under Microgravity

Published by National Aeronautics and Space Administration | National Aeronautics and Space Administration | Metadata Last Checked: October 24, 2025 | Last Modified: 2025-04-23

DLD-1 and MOLT-4 cell lines were cultured in a rotating cell culture system to simulate microgravity and mRNA expression profile in comparison to Static controls. Cells were grown in 10mL rotating vessels in a Rotary Cell Culture System (RCCS) and in 60mm Petri dishes (test control respectively). Two replicates of test (Microgravity) and control (static) each from DLD-1 and MOLT-4 were analyzed by microarray. Simulated microgravity affected the solid tumor cell line DLD-1 markedly which showed a higher percentage of dysregulated genes compared to the hematological tumor cell line MOLT-4. Microgravity affects the cell cycle of DLD-1 cells and disturbs expression of cell cycle regulatory gene networks. Multiple microRNA host genes were dysregulated and significantly mir-22 tumor suppressor microRNA is highly upregulated in DLD-1.

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Complete Metadata

bureauCode	[ "026:00" ]
identifier	nasa_genelab_GLDS-125_um5r-gqim
issued	2021-05-21
landingPage	https://data.nasa.gov/dataset/mrna-expression-profile-in-dld-1-and-molt-4-cancer-cell-lines-cultured-under-microgravity
programCode	[ "026:005" ]
theme	[ "Earth Science" ]