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Surface Water and Porewater Chemistry and Nitrogen Biogeochemistry of Cell 3A, Prado Wetlands, Riverside, CA, USA (2018) (ver. 2.0, April 2025)

Published by U.S. Geological Survey | Department of the Interior | Metadata Last Checked: August 25, 2025 | Last Modified: 20250421
Shallow, unit process open water (UPOW) constructed wetlands effectively remove a range of emerging and traditional water contaminants. Yet, much remains to be learned about internal carbon and nitrogen cycling within these systems and their diel functionality. The current study was conducted at Prado Wetlands in Riverside County, CA, USA in June-September 2018 and focused on light/dark fluctuations in water column and biomat geochemistry. Physical water column parameters were collected using a multiprobe sonde at 15-minute intervals and water samples using an autosampler at 3-hour intervals at the inlet and outlet spillways of the experimental wetland. Porewater samples were collected using an array of Henry samplers. Water samples were analyzed for inorganic anions, cations, metals, total dissolved N, and N and O stable isotopes in nitrate and ammonium. Laboratory microcosm experiments were conducted with slurried wetland biomat to quantify denitrification activity using the acetylene block procedure. In situ column incubations were conducted in light and dark conditions using 15N-sodium nitrite as tracer and analyzed for 15N and 15N content in nitrate, nitrite, ammonium and nitrogen gas to determine rates of N cycling processes within the wetland.

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