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Soil processing method journal article supporting data

Published by U.S. EPA Office of Research and Development (ORD) | U.S. Environmental Protection Agency | Metadata Last Checked: June 27, 2025 | Last Modified: 2016-06-15
This study aimed to optimize a previously used indirect processing protocol, which included a series of washing and centrifugation steps. Optimization of the protocol included: identifying an ideal extraction diluent and evaluating variation in the number of wash steps, variation in the initial centrifugation speed, sonication and shaking mechanisms. The optimized protocol was demonstrated at two laboratories in order to evaluate the recovery of spores from loamy and sandy soils. Data supplied are statistical data and were used to help support conclusions and tables in the journal article. This dataset is associated with the following publication: Silvestri, E., D. Feldhake, D. Griffin, J. Lisle, T. Nichols, S. Shah, A. Pemberton, and F. Schaefer. Optimization of a Sample Processing Protocol for Recovery of Bacillus anthracis Spores from Soil [HS7.52.02 - 514]. JOURNAL OF MICROBIOLOGICAL METHODS. Elsevier Science Ltd, New York, NY, USA, 130: 6-13, (2016).

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