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RNAseq data for eye, gill, intestine, and liver tissues from cultured juvenile black carp (Mylopharyngodon piceus)

Published by U.S. Geological Survey | Department of the Interior | Metadata Last Checked: August 25, 2025 | Last Modified: 20250304
Intact laboratory-cultured juvenile black carp were dissected, and eyes, gills, intestine, and liver from were collected for transcriptomic analysis. Total RNA was extracted using the RNeasy kit (Qiagen), and the RNA quality and quantity were evaluated using the QIAxcel RNA kit. The RNA-Seq library was prepared from mRNA using a TruSeq Stranded mRNA kit (Illumina), and the library was analyzed in paired-read sequencing for 100-bp with 50 million reads per sample in the NovaSeq 6000 S4 Flow Cell system (Illumina). Sequence data are stored on the NCBI Sequence Read Archive (SRA, https://www.ncbi.nlm.nih.gov/sra), under bioproject accession number PRJNA1221815: Transcriptomics in Black carp. Biosample accession numbers are SAMN46752430 through SAMN46752449.

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