RGTM 10169

Published by National Institute of Standards and Technology | National Institute of Standards and Technology | Metadata Last Checked: June 27, 2025 | Last Modified: 2022-07-15 00:00:00

NIST developed a reference material consisting of synthetic fragments of the SARS-CoV-2 virus RNA, which is the target of molecular diagnostic tests. These RNA fragments can assist in the development and validation of RT-qPCR assays for the detection SARS-CoV-2. The RNA fragments are characterized for concentration using digital PCR methods, may be used to assess limits of detection for SARS-CoV-2 assays, and may calibrate other in-house or commercial SARS-CoV-2 controls. This dataset includes data used for RTGM 10169 SARs-Cov-2 Research Grade Test Material validation. The Illumina and ONT sequence data were used to verify the construct sequence.

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Complete Metadata

bureauCode	[ "006:55" ]
identifier	ark:/88434/mds2-2714
issued	2023-02-09
landingPage	https://data.nist.gov/od/id/mds2-2714
language	[ "en" ]
programCode	[ "006:045" ]
theme	[ "Bioscience:Genomic measurements" ]