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Research Article: BMC Immunology
Background
Peroxynitrite is increasingly proposed as a contributor to defence system in marine bivalve. It can be formed by combinaison of superoxide and nitric oxide, and can react with tyrosine residues of proteins giving rise to 3-nitrotyrosine.
Results
The present article describes a competitive ELISA for the measurement of 3-nitrotyrosine contents of plasma proteins from marine bivalves by means of a monoclonal anti 3-nitrotyrosine antibody mouse IgG.
Conclusions
This assay is sensitive enough to determine the amounts of 3-nitrotyrosine in plasma proteins from one animal only.
Using the C-ELISA, we have shown that the phagocytosis of zymosan particles increased the 3-nitrotyrosine levels of plasma proteins from mussel M. galloprovincialis and oyster C. gigas 5.8 and 7.5 times respectively.
Complete Metadata
| bureauCode |
[ "009:25" ] |
|---|---|
| identifier | https://healthdata.gov/api/views/u4nu-pph5 |
| issued | 2025-07-13 |
| landingPage | https://healthdata.gov/d/u4nu-pph5 |
| programCode |
[ "009:033" ] |
| theme |
[ "NIH" ] |
