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Raw light data, coral host genotypic information and zooxanthellae type and relative abundance from coral reefs in the Florida Keys 2015-09-21 to 2016-05-26 (NCEI Accession 0175572)

Published by NOAA National Centers for Environmental Information | National Oceanic and Atmospheric Administration, Department of Commerce | Metadata Last Checked: October 13, 2025 | Last Modified: 2018-09-11T00:00:00.000+00:00
Several parameters were used to assess coral bleaching in the Florida Keys as part of NOAA’s Coral Reef Conservation Program (CRCP). The data described in this package are light, seawater pH, and coral symbiont assemblage data collected from the upper and lower Florida Keys. Light data was collected using a PAR sensor which measures light as photosynthetically active radiation (PAR, umol m-2 s-1). Seawater pH was collected using a SAMI pH sensor or a SeaBird SeaFET pH sensor. These sensors were located at the same sites as the PAR sensors and recorded pH hourly. During peak bleaching (22-25 September 2015), 20 colonies of O. faveolata were sampled with a hammer and chisel from 10 sites (n=200 total coral samples). All corals were sampled from the top of the colony given that symbiont types can change with colony orientation. A small subsample was immediately preserved in 95% ethanol for genetic analysis. Genomic DNA was extracted using the organic extraction protocol described in Rowan and Power (1991). Quantitative PCR (qPCR) assays were used to understand patterns of algal symbiont community structure to detect the presence of background symbiont types. Zooxanthellae type and abundance data are described in this package to understand the relationship between O. faveolata and Symbiodinium to assess coral bleaching responses. Symbiont assemblages in the data set are described by clades A, B, C and D since O. faveolata has been shown to associate with members from all these clades.

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0175572 DNA pH PHOTOSYNTHETIC ACTIVE RADIATION (PAR) PAR Sensor PCR machine pH sensor in situ laboratory analyses NOAA Atlantic Oceanographic and Meteorological Laboratory NOAA Atlantic Oceanographic and Meteorological Laboratory Coral Reef Conservation Program (CRCP) CORAL REEF STUDIES Coastal Waters of Florida Florida Keys National Marine Sanctuary North Atlantic Ocean oceanography AOML Atlantic Oceanographic and Meterological Laboratory DOC/NOAA/OAR/AOML > Atlantic Oceanographic and Meteorological Laboratory, OAR, NOAA, U.S. Department of Commerce 31134 Elucidating the recipe for coral reef resilience in the Florida Keys Genetic Data Sets > PCR/QPCR Detection and Measurements Numeric Data Sets > Biology EARTH SCIENCE > Biosphere > Vegetation > Algae > Reef Monitoring and Assessment EARTH SCIENCE > Biosphere > Vegetation > Algae > Zooxanthellae EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Coral Colony Size and Condition EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Molecular Genetic Analysis > Coral Symbiont Clade Typing EARTH SCIENCE > Oceans > Ocean Chemistry > pH EARTH SCIENCE > Oceans > Ocean Optics > Photosynthetically Active Radiation EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH EARTH SCIENCE > OCEANS > OCEAN OPTICS > PHOTOSYNTHETICALLY ACTIVE RADIATION PAR SENSORS > Photosynthetically Active Radiation Sensors PH METERS > PH METERS COUNTRY/TERRITORY > United States of America > Florida > Lower Florida Keys (24N081W0021) COUNTRY/TERRITORY > United States of America > Florida > Upper Florida Keys (24N080W0018) OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Lower Florida Keys (24N081W0021) OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Upper Florida Keys > Upper Florida Keys (24N080W0018) OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF AMERICA OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF MEXICO M2EAWC

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