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Rare Earth Desorption Test with Citrate and Bicarbonate
LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min.
Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.
Complete Metadata
| bureauCode |
[ "019:20" ] |
|---|---|
| dataQuality | true |
| DOI | 10.15121/1452718 |
| identifier | https://data.openei.org/submissions/7133 |
| issued | 2017-06-01T06:00:00Z |
| landingPage | https://gdr.openei.org/submissions/965 |
| programCode |
[ "019:006" ] |
| projectLead | Holly Thomas |
| projectNumber | LLNL FY17 AOP 2.5.1.12 |
| projectTitle | Extraction of Rare Earth Metals from Geothermal Fluids using Bioengineered Microbes |
| spatial | {"type":"Polygon","coordinates":[[[-122.2433640625,35.15607354867],[-118.3872640625,35.15607354867],[-118.3872640625,39.295415290912],[-122.2433640625,39.295415290912],[-122.2433640625,35.15607354867]]]} |
