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Molecular detection of Coccidioides sp. spores in arid soils and UAS-enabled bioaerosol collection in the San Joaquin Valley, 2024

Published by U.S. EPA Office of Research and Development (ORD) | U.S. Environmental Protection Agency | Metadata Last Checked: June 27, 2025 | Last Modified: 2025-04-16
The goal of this study is to determine if there is an association between aerosolized particulate matter (PM) density, wind speed, temperature, humidity, soil-specific parameters, or other site-specific conditions and Coccidioides detection to better understand fungal spore dispersal within the San Joaquin Valley, CA. Soil and filter samples were concurrently collected using an uncrewed aircraft system (UAS) equipped with bioaerosol samplers flown synchronously at multiple heights. Samples were assessed for the presence of Coccidioides spores per the cocciENV assay (Bowers et al, 2019). Collection sites included Bakersfield, CA and surrounding Kern County (39 total), with 767 samples collected using an interrupted radial transect design.

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