Low dose (0.04 Gy) irradiation (LDR) and hindlimb unloading (HLU) microgravity in mice: retina transcriptomic and epigenomic data

Published by National Aeronautics and Space Administration | National Aeronautics and Space Administration | Metadata Last Checked: October 28, 2025 | Last Modified: 2025-04-23

The purpose of the present study was to evaluate damage in brain and eye in a ground-based model for spaceflight which includes prolonged unloading and low-dose radiation. Low-dose/Low-dose-rate (LDR) gamma-radiation using 57Co plates (0.04 Gy) was delivered whole-body to mature 6-month old female C57BL/6 mice to simulate the radiation component. Anti-orthostatic tail suspension was used to model the unloading fluid shift and physiological stress aspects of the microgravity component. Mice were hindlimb suspended and/or irradiated for 21 days. Tissues were collected at 7 days 1 and 4 months following simulated microgravity. Herein we proposed to use omics-based molecular phenotyping approach for identification and characterization of genomic signatures in multiple organ system associated with low-dose radiation and simulated microgravity.

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data-transformation dna-sequencing-library-construction genelab-rnaseq-data-processing-protocol hindlimb-unloading ionizing-radiation nucleic-acid-extraction nucleic-acid-sequencing rna-sequencing-library-construction sample-collection spike-in-protocol time-of-sample-collection-after-treatment treatment-protocol

Complete Metadata

bureauCode	[ "026:00" ]
identifier	nasa_genelab_GLDS-203_xmr6-iges
issued	2021-05-21
landingPage	https://data.nasa.gov/dataset/low-dose-0-04-gy-irradiation-ldr-and-hindlimb-unloading-hlu-microgravity-in-mice-retina-tr
programCode	[ "026:005" ]
theme	[ "Earth Science" ]