High-volume, label-free imaging for quantifying single-cell dynamics in iPSC colonies

Published by National Institute of Standards and Technology | National Institute of Standards and Technology | Metadata Last Checked: June 27, 2025 | Last Modified: 2023-03-17 00:00:00

This collection is comprised of multiple sets of experiments involving iPSCs with varying dosages of fluorescent light excitation. Samples were imaged every 2 minutes using phase-contrast microscopy for 20+ hours with or without fluorescence excitation. Each experiment was done on different days (i.e. exp0 vs exp1) but with triplicate wells (i.e. exp1-0, exp1-1, exp1-2). Additionally, data used to train our 2D U-Net and 3D U-Net models are included. Detailed information for each dataset can be found in Dataset_Information.xlsx.

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Complete Metadata

bureauCode	[ "006:55" ]
identifier	ark:/88434/mds2-2960
issued	2023-10-02
landingPage	https://data.nist.gov/od/id/mds2-2960
language	[ "en" ]
programCode	[ "006:045" ]
theme	[ "Bioscience:Cell biology" ]