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Development and optimization of species-specific qPCR assays for eDNA detection of the mucket (Ortamannia ligamentina) and spectaclecase (Cumberlandia monodonta) mussels’ male mitotype

Published by U.S. Geological Survey | Department of the Interior | Metadata Last Checked: July 16, 2025 | Last Modified: 20240904
This dataset describes the sensitivity (limit of detection and limit of quantification) and specificity (list of species tested against the assays) of two qPCR assays designed to detect the male mitotype of two freshwater mussel species Ortmanniana ligamentina and Cumberlandia monodonta from water samples (eDNA).

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