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Detection of Multiple Fish Species Using Environmental DNA (eDNA), Alaska 2018

Published by U.S. Geological Survey | Department of the Interior | Metadata Last Checked: September 14, 2025 | Last Modified: 20240802
This release provides results of an environmental DNA (eDNA) study of fish identified in seven Alaskan lakes, three streams, and a positive control. Samples included replicates for multiple sampling dates and locations. The reference list and conserved primer sets to identify species present were developed using publicly available data (https://www.ncbi.nlm.nih.gov/genbank/). Highly conserved primers which could differentiate species of interest were developed for three portions of mtDNA genes (12S, 16S and COI).

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