Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the Fc(gamma)RIa (CD64) Receptor

Published by National Institute of Standards and Technology | National Institute of Standards and Technology | Metadata Last Checked: June 27, 2025 | Last Modified: 2021-02-17 00:00:00

This database gives hydrogen-deuterium exchange mass spectrometry (HDX-MS) data from measurements of three purified IgG1 glycoform samples, predominantly G0F, G2F, and SAF, in isolation and in complexation with the high-affinity receptor, Fc(gamma)RIa (CD64). The IgG1 antibody used in this study, aIL8hFc, is a murine-human chimeric IgG1, which inhibits IL-8 binding to human neutrophils. The working datasets comprise 17,750 deuterium uptake measurements in peptides from the receptor and the light and heavy chains of aIL8hFc obtained during 7 experiments, each comprising three runs of six exchange times. The data illuminate on the hydrogen exchange dynamics in isolated receptor, isolated IgG1 glycoforms, and receptor-IgG1 complexes.

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Complete Metadata

bureauCode	[ "006:55" ]
identifier	ark:/88434/mds2-2365
issued	2021-06-03
language	[ "en" ]
programCode	[ "006:045" ]
theme	[ "Bioscience:Biomaterials", "Bioscience:Proteomics", "Chemistry:Analytical chemistry", "Chemistry:Molecular characterization", "Health:Pharmaceuticals", "Manufacturing:Biomanufacturing", "Materials:Materials characterization" ]