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Data from: Biological Soil Amendments Can Support Survival of Pathogenic and Non-Pathogenic Escherichia coli in Soils and Sporadic Transfer to Romaine Lettuce
<p>Biological soil amendments are an essential input in organic lettuce production. However, these BSAs can introduce or transfer pathogens like <em>Escherichia coli</em> O157:H7 to lettuce in pre-harvest environments. This study evaluated the effect of BSAs on survival of non-pathogenic and pathogenic <em>E. coli</em> in soils and transfer to lettuce. Romaine lettuce was grown with controlled light, temperature, and relative humidity. Soil was amended (side-dressed) with either heat treated poultry pellets (HTPP), HTPP with corn steep liquor (CSL), seabird guano (SBG), SBG with CSL, or left unamended (UA). Soils were co-inoculated with non-pathogenic, rifampicin-resistant <em>E. coli</em> TVS 353 and two chloramphenicol-resistant <em>E. coli</em> O157:H7 isolates (100 mL of 10<sup>6</sup> CFU/mL). <em>E. coli</em> survival over 28 days was evaluated. On day 28, Romaine lettuce was harvested, and presence of <em>E. coli</em> was determined.</p>
Complete Metadata
| bureauCode |
[ "005:18" ] |
|---|---|
| identifier | 10.15482/USDA.ADC/26999374.v1 |
| programCode |
[ "005:040" ] |
| temporal | 2023-08-21/2023-09-18 |