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Data collected from wastewater effluent sampling and on-site fish exposures: trace organic contaminant concentrations, bacteria concentrations, and fish responses

Published by U.S. Geological Survey | Department of the Interior | Metadata Last Checked: July 15, 2025 | Last Modified: 20250203
This dataset consists of trace organic contaminant concentrations and bacteria counts in wastewater effluents, and observed responses in fish exposed to effluents. The study was conducted from April to September 2022 at a facility located in Hutchinson, Minnesota. Nine pairs of primary-treated, activated sludge secondary treated, and membrane bioreactor secondary treated wastewater were characterized for select trace organic contaminants (pharmaceuticals, pesticides and pesticide degradates, hormones, alkylphenols, and bisphenols) and bacterial (total coliform, Escherichia coli) counts. Two 21-day exposures were conducted on-site in which fathead minnows were exposed to activated sludge secondary treated effluent and membrane bioreactor secondary treated effluent. Effluent samples were collected once per month in April, August, and September, and once weekly during the on-site exposures.

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