CottonGen Primer3

<p>Primer3web version 4.0.0 allows users to pick PCR primers from a DNA sequence based on criteria including:</p> <ul> <li> <p>oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities,</p> </li> <li> <p>PCR product size,</p> </li> <li> <p>positional constraints within the source (template) sequence, and</p> </li> <li> <p>possibilities for ectopic priming (amplifying the wrong sequence)</p> </li> <li> <p>many other constraints.</p> </li> </ul> <p>All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.</p> <div><br>Resources in this dataset:</div><br><ul><li><p>Resource Title: Website Pointer for CottonGen Primer3.</p> <p>File Name: Web Page, url: <a href="https://www.cottongen.org/tools/primer3" target="_blank">https://www.cottongen.org/tools/primer3</a> </p></li></ul>

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