Analytical Performance of Direct-to-Consumer Gut Microbiome Testing Services

Published by National Institute of Standards and Technology | National Institute of Standards and Technology | Metadata Last Checked: July 14, 2025 | Last Modified: 2024-06-05 00:00:00

This repository contains raw and intermediate data files as well as analysis code from the manuscript "Evaluating the Analytical Performance of Direct-to-Consumer Gut Microbiome Testing Services". In this analysis a pilot version of the homogenized whole human stool reference material was submitted as a sample to commercial direct to consumer microbiome testing companies. In addition, NIST conducted in house characterization by 16S amplicon sequencing and whole metagenomic sequencing (WMS) on the same sample. Raw sequencing data from the sequencing providers was not obtained for this study; however sequencing files (fastq) both 16S amplicon sequencing analysis and whole metagenomic sequencing (WMS) conducted at NIST are included. In addition, the fastq files from other donors that contributed to the pilot material and included in this analysis to represent biological diversity are also included in this record.

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Complete Metadata

bureauCode	[ "006:55" ]
identifier	ark:/88434/mds2-3348
issued	2024-06-20
landingPage	https://data.nist.gov/od/id/mds2-3348
language	[ "en" ]
programCode	[ "006:045" ]
replaces	ark:/88434/mds2-3348
theme	[ "Bioscience:Microbial measurements", "Health:Precision medicine" ]