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16S and ITS amplicon sequencing of reconstructed wetland soil metagenomes

Published by U.S. EPA Office of Research and Development (ORD) | U.S. Environmental Protection Agency | Metadata Last Checked: June 27, 2025 | Last Modified: 2021-04-23
Experiments tested if changes in soil microbiomes within reconstructed freshwater wetlands were associated with exposure to elevated Cu concentrations originating from immersed Micronized Copper Azole (MCA) treated wood stakes. Soil samples were collected for amplicon sequencing to quantify responses in microbial communities at 15 timepoints, spanning 2 simulated seasonal dry downs, for up to 678 days. This dataset is associated with the following publication: Reichman, J., M. Johnson, P. Rygiewicz, B. Smith, M. Bollman, M. Storm, G. King, and C. Andersen. Focused Microbiome Shifts in Reconstructed Wetlands Correlated with Elevated Copper Concentrations Orginating from Micronized Copper Azole- Treated Wood. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY. Society of Environmental Toxicology and Chemistry, Pensacola, FL, USA, 40(12): 3351-3368, (2021).

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