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Found 356 dataset(s) matching "PCR".
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<p>Deer keds are blood-feeding flies from which several human and animal pathogens have been detected, including the causative agent of Lyme Disease (<em>Borrelia burgdorferi</em>). Cervids, which...
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Early diagnosis of wound colonisation or prediction of wound sepsis provides an opportunity for therapeutic intervention. There is need for qualitative and quantitative tests that are more rapid...
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<p>Amplicon sequencing utilizing next-generation platforms has significantly transformed how research is conducted, specifically microbial ecology. However, primer and sequencing platform biases...
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<p>Data are the individual group values for oral and cloacal virus shedding and antibody titers for reach treatment group from: Mo et al., The pathogenicity and transmission of live bird market...
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Background SP100 is a nuclear protein that displays a number of alternative splice variants. In Old World monkeys, apes and humans one of these variants is extended by a retroprocessed...
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Gene expression data from the Fluidigm qRT-PCR arrays was analyzed in R (v3.6.1; R Foundation for Statistical Computing, 2019). Prior to processing through the tcpl package, each qRT-PCR primer...
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Influenza A viruses are one of the most significant viral groups globally with substantial impacts on human, domestic animal and wildlife health. Wild birds are the natural reservoirs for these...
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Environmental DNA (eDNA) detection of invasive species can be used to delimit occupied ranges and estimate probabilities to inform management decisions. Environmental DNA is shed into the...
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Background Methods for the extraction of DNA from filamentous fungi are frequently laborious and time consuming because most of the available protocols include maceration in liquid...
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Background Blood group genotyping is increasingly utilized for prenatal diagnosis and after recent transfusions, but still lacks the specificity of serology. In whites, the presence of...
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<p dir="ltr">In a 2023 survey evaluating conifers with <i>Rosellinia </i>infections, five <i>Pestalotiopsis</i>-like fungal endophytes were isolated from plant samples obtained from Maine, New...
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These data were compiled to provide land-management relevant information on the native and nonnative subspecies (subsp.) of Phragmites australis (subsp. americanus and subsp. australis,...
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From 2010-2018 we investigated the occurrence of avian influenza virus in wild birds in Iceland. A total of 6635 swabs samples were collected from wild birds or fecal material directly associated...
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Background Oxidative stress, resulting in a marked increase in the level of oxygen free radicals (OFR), has been implicated in the etiology of diabetic neuropathy (DN). Antioxidant...
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Background Abnormal collagen metabolism is thought to play an important role in the development of primary inguinal hernia. This is underlined by detection of altered collagen metabolism...
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RT-qPCR is used world-wide to test and trace the spread of SARS-CoV-2. “Extraction-less” or “direct” RT-PCR is an open-access qualitative method for SARS-CoV-2 detection from nasopharyngeal (NP)...
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Background PCR amplification of target molecules involves sequence specific primers that flank the region to be amplified. While this technique is generally routine, its applicability...
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Environmental DNA (eDNA) detection is a rapidly expanding technique used to non-invasively detect cryptic, low density, or logistically difficult-to-study species, such as imperiled manatees....
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Avian botulism toxicity is a common cause of death to water and shore birds that live near or migrate through Lake Michigan. The botulism neuro-toxin type E (bontE) gene is responsible for the...
