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Found 84 dataset(s) matching "Illumina sequencing".
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<p>House flies (<em>Musca domestica</em> L.) are vectors of human and animal pathogens at livestock operations. Microbial communities in flies are acquired from, and correlate with, their local...
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<p dir="ltr">Adult house flies (<i>Musca domestica</i> L.) are important reservoirs and mechanical vectors of bacteria in livestock operations. House fly bacterial communities are influenced by...
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This study was performed to determine the role of adrenal-derived stress hormones in mediating brain effects after ozone exposure. Sham and adrenalectomized rats were exposed to air or ozone for 4...
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Methods: RNA-seq libraries were prepared using the Illumina TruSeq RNA kit and the TrueSeq method was employed for mRNA enrichment. The libraries were quantified and samples were multiplexed in...
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Efficient generation of cardiomyocytes from human pluripotent stem cells is critical for their regenerative applications. Microgravity and 3D culture can profoundly modulate cell proliferation and...
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Methods: RNA-seq libraries were prepared using the Illumina TruSeq RNA kit and the TrueSeq method was employed for mRNA enrichment. The libraries were quantified and samples were multiplexed in...
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Low RNA yield and quality limit use of formalin-fixed paraffin-embedded (FFPE) tissue samples for genomic analyses. In this study, we evaluated methods to demodify RNA highly fragmented and...
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<p>The longhorned tick, <em>Haemaphysalis longicornis</em>, feeds upon a wide range of bird and mammalian hosts. Mammalian hosts include cattle, deer, sheep, goats, humans, and horses. This tick...
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se of archival resources has been limited to date by inconsistent methods for genomic profiling of degraded RNA from formalin-fixed paraffin-embedded (FFPE) samples. RNA-seq offers a novel way to...
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The precisionFDA Truth Challenge V2 aimed to assess the state-of-the-art of variant calling in difficult-to-map regions and the Major Histocompatibility Complex (MHC). Starting with FASTQ files,...
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H. verticillata samples collected from numerous watersheds from 2014 to 2020 were screened for the presence of A. hydrillicola. After isolation of A. hydrillicola from environmental samples and...
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While it has been shown that astronauts suffer immune disorders after spaceflight, the underlying causes are still poorly understood and there are many variables to consider when investigating the...
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To assess the variability of low-abundance oligonucleotide detection across sample matrices, we spiked DNA reference standards (meta sequins) into replicate wastewater DNA extracts at...
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<p>Soil microorganisms play essential roles in soil organic matter dynamics and nutrient cycling in agroecosystems and have been used as soil quality indicators. The response of soil microbial...
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This study presents the isolation of exosomes and RNA from 20 samples of white-tailed deer serum using a commercially available kit, incorporating optimizations for RNA yield through two elutions...
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In order to determine the innate microbial community of shale gas reservoirs and how they are impacted by hydraulic fracturing, this study analyzed biomass collected from produced water and rock...
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The data consists of sequence reads generated from an Illumina MiSeq. This dataset is associated with the following publication: Ruth, N., N. Brinkman, M. Jahne, P. Chain, S. Keely, and M....
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The dataset contains the full fathead minnow (FHM) reference genome and annotation data as well all DNA sequencing data (PacBio long reads and Illumina paired-end reads) and RNA-seq data that were...
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<p>The Sequence Read Archive (SRA) stores raw sequencing data from the next generation of sequencing platforms including Roche 454 GS System®, Illumina Genome Analyzer®, Applied Biosystems SOLiD®...
