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Found 356 dataset(s) matching "PCR".
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Background Chlamydia pneumoniae infection has been detected by serological methods, but PCR is gaining more interest. A number of different PCR assays have been developed and some are...
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Background In a variant of the standard PCR reaction termed bridging, or jumping, PCR the primer-bound sequences are originally on separate template molecules. Bridging can occur if, and...
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Background Prompt laboratory diagnosis of Herpes simplex virus (HSV) infection facilitates patient management and possible initiation of antiviral therapy. In our laboratory, which...
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There is a need for development of an analytical method for rapid detection of SARS-CoV-2 virus which is causing the COVID-19 pandemic. Currently available traditional tissue/cell culture-based...
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Background Suppression Subtractive Hybridization PCR (SSH PCR) is a sophisticated cDNA subtraction method to enrich and isolate differentially expressed genes. Despite its popularity,...
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<p>Some U.S bioethanol plants use a blend of maize and sorghum grains as feedstocks depending on availability and price. Knowledge of the grain composition of milled feedstock is beneficial for...
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Droplet digital PCR (ddPCR) is being advocated as a reference method to measure rare genomic targets. It has consistently been proven to be more sensitive and direct at discerning copy numbers of...
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Background Leptospirosis is an emerging infectious disease. The differential diagnosis of leptospirosis is difficult due to the varied and often "flu like" symptoms which may result in a...
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Background Rapid diagnosis and correct treatment of cases are the main objectives of control programs in malaria-endemic areas. Methods and results ...
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The entire process of reverse transcription-polymerase chain reaction (RT-PCR) is simplified by using oligonucleotide-immobilized microplates made of, e.g., polypropylene, to which...
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Background We describe an alternative method to determine mRNA half-life (t1/2) based on the Real-Time RT-PCR procedure. This approach was evaluated by using the β-actin gene as a...
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Background Norwalk-like viruses are the most common cause of gastroenteritis outbreaks and sporadic cases of vomiting and diarrhoea. In healthy individuals infection is often mild and...
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Bacillus species and B. anthracis presence/absence data were determined in 4,770 soil samples collected across the contiguous United States, in cooperation with the U.S. Environmental Protection...
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The emergence of ophidiomycosis (or snake fungal disease) in snakes has prompted increased awareness of the potential impacts of fungal infections on wild reptile populations. Yet, aside from...
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<p>A GeXP multiplex, RT-PCR assay was developed and optimized that simultaneously measures expression of a suite of immune-relevant genes in rainbow trout (<em>Oncorhynchus mykiss</em>),...
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Provides droplet digital polymerase chain reaction (ddPCR) and quantitative PCR (qPCR) measurements of adenovirus and norovirus in three decentralized wastewater and graywater collection systems....
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Background Multiple endocrine neoplasias type 2A (MEN 2A) is a dominantly inherited cancer syndrome. Missence mutations in the codon encoding cysteine 634 of the ret proto-oncogene have...
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Dataset includes repeated measures of the National Institute of Standards and Technology Standard Reference Material 2917 (SRM 2917) using an Enterococcus digital PCR (dPCR) assay. “This...
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We developed and validated conventional and quantitative real-time PCR assays for the detection of DNA from the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of...
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This dataset includes the standard curves for ICCRTqPCR to convert the assay quantities to the concentrations of infectious viruses and all the calculations on inactivation rate constants. Also,...
